1. All of the following are benefits of using a flow cytometer except:

A. counting a large number of cells quickly.

B. isolating a specific cell type.

C. requiring the overnight culturing of samples.

D. identifying the remission of diseases.

2. All of the points that appear on the graph display output of a flow cytometer represent the total number of bacteria cells of a specific type present in a sample.

A. true

B. false

3. What is the typical minimum cell count that demonstrates Salmonella contamination in a 0.25 mL sample run on a flow cytometer?

A. 10

B. 25

C. 40

D. 50

4. The graph display on the output of a flow cytometer typically has which type of axis scaling?

A. logarithmic

B. arithmetic

C. temporal

D. none of these

5. Which of the following is not a typical component of a flow cytometer?

A. laser

B. emission detector

C. output display

D. mass spectrometer

6. The readout of a flow cytometer has the following data: Salmonella cell count = 120, with 20% of cells residing in the predefined boxed area of the graph. How should this sample be labeled?

A. contaminated

B. not contaminated

C. cannot be determined

7. Which of the following is/are excitation device(s) in a flow cytometer?

A. laser, only

B. impendence-based system, only

C. both laser and impedance-based systems

D. neither laser nor impedance-based systems

8. The readout of a flow cytometer has the following data: Salmonella cell count = 500, with 30% of cells residing in the predefined boxed area of the graph. How should this sample be labeled?

A. contaminated

B. not contaminated

C. cannot be determined

Lesson 1 Going with the Flow: Going with the Flow (Advanced)